DIFFERENTIAL RESPONSIVENESS OF PROSTATIC ACID PHOSPHATASE AND PROSTATE-SPECIFIC ANTIGEN mRNA TO ANDROGEN IN PROSTATE CANCER CELLS

Authors

  • Ming-Fong Lin,

    Corresponding author
    1. Department of Biochemistry and Molecular Biology, College of Medicine
    2. Section of Urologic Surgery, College of Medicine
    3. Eppley Cancer Institute, University of Nebraska Medical Center
    4. Omaha VA Hospital, Omaha, NE, 68198, U.S.A.
      To whom correspondence should be addressed: Ming-Fong Lin, Ph.D., Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, 984525 Nebraska Medical Center, Omaha, NE 68198-4525, U.S.A. E-mail:mlin@unmc.edu
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  • Ming-Shyue Lee,

    1. Department of Biochemistry and Molecular Biology, College of Medicine
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  • Renee Garcia-Arenas,

    1. Department of Biochemistry and Molecular Biology, College of Medicine
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  • Fen-Fen Lin

    1. Department of Biochemistry and Molecular Biology, College of Medicine
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To whom correspondence should be addressed: Ming-Fong Lin, Ph.D., Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, 984525 Nebraska Medical Center, Omaha, NE 68198-4525, U.S.A. E-mail:mlin@unmc.edu

Abstract

Androgens regulate the expression of both human prostatic acid phosphatase (PAcP) and prostate-specific antigen (PSA), two major prostate epithelium-specific differentiation antigens. Due to the important role of these two enzymes as prostate epithelium differentiation markers, we investigated their regulation of expression at the mRNA level in LNCaP human prostate carcinoma cells. Interestingly, phenol red, a pH indicator in the culture medium, promoted cell growth. To eliminate this non-specific effect, a phenol red-free, steroid-reduced medium was utilized. When high-density cells were grown in that medium, 5α-dihydrotestosterone (DHT) suppressed PAcP but stimulated PSA. However, tumor promoter phorbol ester 12- o -tetradecanoyl phorbol-13-acetate (TPA) functioned as a potent inhibitor of both PAcP and PSA expression. Prolonged treatment with DHT as well as TPA resulted in a similar down-regulation of protein kinase C and cellular PAcP activities. Thus, the levels of PAcP and PSA mRNA are differentially regulated by androgens in LNCaP cells.

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