2,7-DICHLOROFLUORESCIN OXIDATION AND REACTIVE OXYGEN SPECIES: WHAT DOES IT MEASURE?
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 2000 International Federation for Cell Biology
Cell Biology International
Volume 24, Issue 10, pages 757–760, October 2000
How to Cite
Jakubowski, W. and Bartosz, G. (2000), 2,7-DICHLOROFLUORESCIN OXIDATION AND REACTIVE OXYGEN SPECIES: WHAT DOES IT MEASURE?. Cell Biology International, 24: 757–760. doi: 10.1006/cbir.2000.0556
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Received 5 August 1999; Accepted 17 April 2000
- Cited By
- oxidative stress;
- hydrogen peroxide;
- reactive oxygen species;
Intracellular 2,7-dichlorofluorescin (H2DCF) oxidation is often used to measure the production of reactive oxygen species (ROS) within cells. The rate of H2DCF oxidation depends on the concentration of glutathione, which is an alternative target for ROS. Our results suggest that increased rate of H2DCF oxidation be interpreted as an indication of general oxidative stress due to a variety of reasons, including depletion of antioxidants, rather than as a specific proof of augmented ROS formation.