AUTOMATED DETECTION OF MORPHOLOGICAL ALTERATIONS DURING APOPTOSIS

Authors

  • Thawatchai Lertworasirikul,

    1. Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok, Thailand
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  • Ahnond Bunyaratvej,

    Corresponding author
    1. Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
      To whom correspondence should be addressed: Professor Dr Ahnond Bunyaratvej, Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Rama 6 Road, Bangkok, 10400, Thailand. E-mail:raaby@mahidol.ac.th
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  • Fumio Kubota

    1. 4-4-4 Takatsukadai Nishi-ku, Kobe, 651-2271, Japan
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To whom correspondence should be addressed: Professor Dr Ahnond Bunyaratvej, Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Rama 6 Road, Bangkok, 10400, Thailand. E-mail:raaby@mahidol.ac.th

Abstract

A convenient means of measuring apoptosis is described using an automated analyzer (MF-1; Sysmex), which normally provides rapid measurement of malarial parasites. By means of this MF-1 procedure, apoptotic cells exhibited characteristic changes of light scatter (size) and fluorescence (DNA content) relating to cell shrinkage and nuclear fragmentation of apoptosis.

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