INVOLVEMENT OF PI 3-KINASE, PKA AND PKC IN PDGF- AND TGF-β-MEDIATED PREVENTION OF 2-DEOXY-D-RIBOSE-INDUCED APOPTOSIS IN THE INSECT CELL LINE, IPLB-LdFB

Authors

  • Enzo Ottaviani,

    Corresponding author
    1. Department of Animal Biology, University of Modena and Reggio Emilia, via Campi, 213/0 41100, Modena, Italy
      To whom correspondence should be addressed: Prof. Enzo Ottaviani, Department of Animal Biology, Via Campi, 213/0 41100 Modena, Italy. E-mail:ottaviani.enzo@unimo.it
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  • Daniela Barbieri,

    1. Department of Animal Biology, University of Modena and Reggio Emilia, via Campi, 213/0 41100, Modena, Italy
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  • Davide Malagoli,

    1. Department of Animal Biology, University of Modena and Reggio Emilia, via Campi, 213/0 41100, Modena, Italy
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  • Dimitris Kletsas

    1. Institute of Biology, National Center for Scientific Research ‘Demokritos’ Athens, 153 10, Greece
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To whom correspondence should be addressed: Prof. Enzo Ottaviani, Department of Animal Biology, Via Campi, 213/0 41100 Modena, Italy. E-mail:ottaviani.enzo@unimo.it

Abstract

Activation of phosphatidylinositol (PI) 3-kinase, protein kinase A (PKA) and protein kinase C (PKC) is associated with the survival effect elicited by PDGF-AB and TGF-β1 against the apoptotic inducer 2-deoxy-D-ribose (dRib) in the fat body cell line, IPLB-LdFB, from the insect Lymantria dispar. dRib induces apoptosis and provokes mitochondrial membrane depolarization (MMD). The antioxidant N -acetyl-L-cysteine annuls only the first effect. These findings suggest that apoptosis and MMD are provoked by two different mechanisms, and that dRib induces apoptosis by oxidative stress.

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