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Keywords:

  • cell differentiation;
  • vitamin D3;
  • cell cycle

Human promyelocytic leukaemic cells, HL-60, arrested in mitosis by nocodazole were released in the presence of 1α,25-dihydroxyvitamin D3 and thymidine or hydroxyurea. Cells moved from early G1 period to the G1/S boundary and differentiated. Furthermore, cells arrested at the G1/S boundary by double thymidine block were released, with 1α,25-dihydroxyvitamin D3 being added at the end of DNA synthesis. Under the latter conditions, differentiated cells developed, indicating that DNA synthesis is not required for cell differentiation.