EFFECT OF FGF-1 AND FGF-2 ON VEGF BINDING TO HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS

Authors

  • Jun-Hui Chen,

    Corresponding author
    1. Pharmaceutic Biotechnology Key Laboratory, Department of Biochemistry, Nanjing University, Nanjing, 210093, P.R. China
    2. W. Alton Jones Cell Science Center, Inc. 10 Old Barn Road, Lake Placid, NY, 12946, U.S.A.
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  • Xin-Chang Wang,

    1. Pharmaceutic Biotechnology Key Laboratory, Department of Biochemistry, Nanjing University, Nanjing, 210093, P.R. China
    2. W. Alton Jones Cell Science Center, Inc. 10 Old Barn Road, Lake Placid, NY, 12946, U.S.A.
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  • Mikio Kan,

    1. W. Alton Jones Cell Science Center, Inc. 10 Old Barn Road, Lake Placid, NY, 12946, U.S.A.
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  • J. Denry Sato

    1. W. Alton Jones Cell Science Center, Inc. 10 Old Barn Road, Lake Placid, NY, 12946, U.S.A.
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Department of Biochemistry, Nanjing University, Nanjing, 210093, P.R. China. E-mail: cjhwxcxx@public1.ptt.js.cn

Abstract

When FGF-1 or FGF-2 and VEGF were added together, the mitogenic effect of FGF-1 or FGF-2 and VEGF on HUVEC was additive. However, when HUVECs were preincubated for 2 days with 10ng/ml FGF-1 in the absence of VEGF, the Scatchard plot of [125I]VEGF binding sites was shifted to the right: both affinity classes of VEGF binding sites were equally affected, such that the total number of sites increased twofold. It is suggested that this type of interaction may be related to tumor angiogenesis and wound repair.

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