BIFUNCTIONAL EFFECTS OF HEPARIN-BINDING PROTEIN HBp17 ON DNA SYNTHESIS IN CELLS

Authors

  • Jun-Hui Chen,

    Corresponding author
    1. Pharmaceutic Biotechnology Key Laboratory, Department of Biochemistry, Nanjing University, Nanjing, 210093, P.R. China
    2. W. Alton Jones Cell Science Center, Inc. 10 Old Barn Road, Lake Placid, NY, 12946, U.S.A.
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  • Xin Chang Wang,

    1. Pharmaceutic Biotechnology Key Laboratory, Department of Biochemistry, Nanjing University, Nanjing, 210093, P.R. China
    2. W. Alton Jones Cell Science Center, Inc. 10 Old Barn Road, Lake Placid, NY, 12946, U.S.A.
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  • J. Denry Sato

    1. W. Alton Jones Cell Science Center, Inc. 10 Old Barn Road, Lake Placid, NY, 12946, U.S.A.
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Pharmaceutic Biotechnology Key Laboratory, Department of Biochemistry, Nanjing University, Nanjing, 210093, P.R. China. Fax: 0086-025-3592510. E-mail: cjhwxcxx@public1.ptt.js.cn

Abstract

A 17kD heparin-binding protein (HBp17) has a biphasic dose-dependent effect on DNA synthesis in 3T3 cells. Maximal stimulation of DNA synthesis occurs at 8ng/ml HBp17, but a half-maximal inhibition occurs at ∼500ng/ml. This inhibition can easily be reversed by addition of 400pg/ml aFGF or 100pg/ml bFGF, whereas EGF had no effect. This biphasic action of HBp17 was also seen in human umbilical vein endothelial cells (HUVEC), whereas it was not found in the malignant cell line, A431-AJC. The functional relationship between HBp17 and FGF is discussed.

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