cGMP-REGULATED STORE-OPERATED CALCIUM ENTRY IN HUMAN HEPATOMA CELLS

Authors

  • J. L. Jiang,

    1. Epithelial Cell Biology Research Center, Department of Physiology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong
    2. Cell Engineering Laboratory, The Fourth Military Medical University, Xi'an, P.R. China
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  • M. K. Yu,

    1. Epithelial Cell Biology Research Center, Department of Physiology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong
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  • Z. N. Chen,

    1. Cell Engineering Laboratory, The Fourth Military Medical University, Xi'an, P.R. China
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  • H. C. Chan

    Prof., Corresponding author
    1. Epithelial Cell Biology Research Center, Department of Physiology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong
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To whom correspondence should be addressed: Prof. H. C. Chan, Epithelial Cell Biology Research Center, Department of Physiology, Faculty of Medicine, Chinese University of Hong Kong, Shatin, Hong Kong. Tel: (852) 2609 6839; Fax: (852) 2603 5022; hsiaocchan@cuhk.edu.hk

Abstract

This study aimed to investigate cGMP-regulated store-operated Ca2+entry in human 7721 hepatoma cells. [Ca2+]iwas measured using Fura2/AM. After incubation of the cells with 4μm thapsigargin, Ca2+entry was evoked by application of 1m m Ca2+to extracellular solution and was blocked by 3m m Ni2+, indicating the presence of store-operated Ca2+entry in human 7721 hepatoma cell line. Application of 8-Br-cGMP reduced the [Ca2+]iin hepatoma 7721 cells by 80%. These data demonstrated for the first time that store-operated Ca2+entry pathway is present in human hepatoma cells, which is regulated by cGMP.

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