Equine satellite cell clone SE-11 and ovine satellite cell clone I1were evaluated for expression of myosin heavy chain, myogenin, desmin, and muscle-specific actin over a 240h period in culture. An enzyme-linked immunoculture assay (ELICA) was capable of detecting these proteins at all time points evaluated. A linear relationship was demonstrated between the natural logarithm of the absorbance values (corrected for cell number) from the ELICA and percent fusion in both SE-11 and I1cultures. The r2values for SE-11 cultures were: desmin 0.82, muscle actin 0.81, myogenin 0.78, and myosin 0.70. The r2values for I1cultures were: desmin 0.77, muscle actin 0.72, myogenin 0.70, and myosin 0.61. Our confocal results support the idea that differences exist between species in the differentiation dynamics of satellite cells. Further, these data suggest that the ELICA may be applied to previously conducted experiments, enabling additional data to be obtained with relation to muscle protein expression.