CHANGES IN FUSED CELLS INDUCED BY HVJ (SENDAI VIRUS): REDISTRIBUTION OF CYTOPLASMIC ORGANELLES AND CYTOSKELETAL REORGANIZATION

Authors

  • Etsuko Hirayama,

    1. Institute of Molecular and Cellular Biology for Pharmaceutical Sciences, Kyoto Pharmaceutical University, 1, Shichonocho, Misasagi, Yamashina-ku, Kyoto, 607-8412, Japan
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  • Masako Ama,

    1. Institute of Molecular and Cellular Biology for Pharmaceutical Sciences, Kyoto Pharmaceutical University, 1, Shichonocho, Misasagi, Yamashina-ku, Kyoto, 607-8412, Japan
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  • Ken-Ichiro Takahashi,

    1. Sumitomo Pharmaceuticals Co., Ltd. Osaka, Japan
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  • Akihiro Hiraki,

    1. Institute of Molecular and Cellular Biology for Pharmaceutical Sciences, Kyoto Pharmaceutical University, 1, Shichonocho, Misasagi, Yamashina-ku, Kyoto, 607-8412, Japan
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  • Jeman Kim

    Corresponding author
    1. Institute of Molecular and Cellular Biology for Pharmaceutical Sciences, Kyoto Pharmaceutical University, 1, Shichonocho, Misasagi, Yamashina-ku, Kyoto, 607-8412, Japan
      To whom correspondence should be addressed: Jeman Kim, Fax: +81-075-595-4798; kim@mb.kyoto-phu.ac.jp
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To whom correspondence should be addressed: Jeman Kim, Fax: +81-075-595-4798; kim@mb.kyoto-phu.ac.jp

Abstract

To understand the relationship between the location of organelles and cellular function, we examined the dynamic state of cytoplasmic organelles and cytoskeleton in polynuclear Ehrlich ascites tumor (EAT) cells fused with hemagglutinating virus of Japan (HVJ; Sendai virus) by confocal laser scanning microscopy. Irregular fused cells gradually became spherical during culture, and nuclei and mitochondria were redistributed in the fused cell; nuclei formed a cluster surrounded by mitochondria. F-actin, vimentin, and microtubules were also reorganized with the redistribution of cell organelles. Further, when the morphological change was inhibited by L4-1, a chlorophyll-like substance derived from silkworm faeces, or pyropheophorbide-a, the arrangement of organelles and cytoskeleton remained disturbed, suggesting that the movement of the cytoskeleton is closely associated with cell shape and the distribution of cytoplasmic organelles.

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