Intracellular localization of an active green fluorescent protein-tagged Pho84 phosphate permease in Saccharomyces cerevisiae

Authors

  • Jens Petersson,

    1. Department of Engineering and Natural Sciences, Växjö University, 351 95 Växjö, Sweden
    2. Department of Biochemistry, Stockholm University, 106 91 Stockholm, Sweden
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  • Johanna Pattison,

    1. Department of Engineering and Natural Sciences, Växjö University, 351 95 Växjö, Sweden
    2. Department of Biochemistry, Stockholm University, 106 91 Stockholm, Sweden
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  • Arthur L. Kruckeberg,

    1. E.C. Slater Institute/BioCentrum Amsterdam, University of Amsterdam, Plantage Muidergracht 12, 1018 TV Amsterdam, The Netherlands
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  • Jan A. Berden,

    1. E.C. Slater Institute/BioCentrum Amsterdam, University of Amsterdam, Plantage Muidergracht 12, 1018 TV Amsterdam, The Netherlands
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  • Bengt L. Persson

    Corresponding author
    1. Department of Engineering and Natural Sciences, Växjö University, 351 95 Växjö, Sweden
    2. Department of Biochemistry, Stockholm University, 106 91 Stockholm, Sweden
    • Corresponding author. Fax: (46) (470) 70 87 56

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Abstract

Green fluorescent protein (GFP) from Aequorea victoria was used as an in vivo reporter protein when fused to the carboxy-terminus of the Pho84 phosphate permease of Saccharomyces cerevisiae. Both components of the fusion protein displayed their native functions and revealed a cellular localization and degradation of the Pho84-GFP chimera consistent with the behavior of the wild-type Pho84 protein. The GFP-tagged chimera allowed for a detection of conditions under which the Pho84 transporter is localized to its functional environment, i.e. the plasma membrane, and conditions linked to relocation of the protein to the vacuole for degradation. By use of the methodology described, GFP should be useful in studies of localization and degradation also of other membrane proteins in vivo.

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