Ralstonia metallidurans, a bacterium specifically adapted to toxic metals: towards a catalogue of metal-responsive genes

Authors

  • Max Mergeay,

    Corresponding author
    1. Laboratories for Microbiology and Radiobiology, Belgium Nuclear Research Center, SCK/CEN, B-2400 Mol, Belgium
    2. Environmental Technology Expertise Center, Flemish Institute for Technological Research, Vito, B-2400 Mol, Belgium
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  • Sébastien Monchy,

    1. Laboratories for Microbiology and Radiobiology, Belgium Nuclear Research Center, SCK/CEN, B-2400 Mol, Belgium
    2. Unité d’étude de la Conformation des Macromolécules Biologiques, Université Libre de Bruxelles, Brussels, Belgium
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  • Tatiana Vallaeys,

    1. Laboratories for Microbiology and Radiobiology, Belgium Nuclear Research Center, SCK/CEN, B-2400 Mol, Belgium
    2. Département de Microbiologie, Institut National de la Recherche Agronomique (INRA), Jouy-en-Josas, France
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  • Vanessa Auquier,

    1. Centre de Biologie structurale et de Bioinformatique: Structure et Fonction des Membranes Biologiques, Université Libre de Bruxelles, Brussels, Belgium
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  • Abderrafi Benotmane,

    1. Laboratories for Microbiology and Radiobiology, Belgium Nuclear Research Center, SCK/CEN, B-2400 Mol, Belgium
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  • Philippe Bertin,

    1. Laboratories for Microbiology and Radiobiology, Belgium Nuclear Research Center, SCK/CEN, B-2400 Mol, Belgium
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  • Safiyh Taghavi,

    1. Environmental Technology Expertise Center, Flemish Institute for Technological Research, Vito, B-2400 Mol, Belgium
    2. Biology Department, Bldg. 463, Brookhaven National Laboratories (BNL), Upton, NY 11973-5000, USA
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  • John Dunn,

    1. Biology Department, Bldg. 463, Brookhaven National Laboratories (BNL), Upton, NY 11973-5000, USA
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  • Daniel van der Lelie,

    1. Environmental Technology Expertise Center, Flemish Institute for Technological Research, Vito, B-2400 Mol, Belgium
    2. Biology Department, Bldg. 463, Brookhaven National Laboratories (BNL), Upton, NY 11973-5000, USA
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  • Ruddy Wattiez

    1. Service de Chimie Biologique, Université de Mons-Hainaut, Mons, Belgium
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*Corresponding author. Tel.:+32 (14) 332727; Fax:+32 (14) 314793, E-mail address:mmergeay@sckcen.be

Abstract

Ralstonia metallidurans, formerly known as Alcaligenes eutrophus and thereafter as Ralstonia eutropha, is a β-Proteobacterium colonizing industrial sediments, soils or wastes with a high content of heavy metals. The type strain CH34 carries two large plasmids (pMOL28 and pMOL30) bearing a variety of genes for metal resistance. A chronological overview describes the progress made in the knowledge of the plasmid-borne metal resistance mechanisms, the genetics of R. metallidurans CH34 and its taxonomy, and the applications of this strain in the fields of environmental remediation and microbial ecology. Recently, the sequence draft of the genome of R. metallidurans has become available. This allowed a comparison of these preliminary data with the published genome data of the plant pathogen Ralstonia solanacearum, which harbors a megaplasmid (of 2.1 Mb) carrying some metal resistance genes that are similar to those found in R. metallidurans CH34. In addition, a first inventory of metal resistance genes and operons across these two organisms could be made. This inventory, which partly relied on the use of proteomic approaches, revealed the presence of numerous loci not only on the large plasmids pMOL28 and pMOL30 but also on the chromosome. It suggests that metal-resistant Ralstonia, through evolution, are particularly well adapted to the harsh environments typically created by extreme anthropogenic situations or biotopes.

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