What students must know about the determination of enzyme kinetic parameters
Article first published online: 30 JUN 2010
Copyright © 1999 International Union of Biochemistry and Molecular Biology, Inc.
Volume 27, Issue 2, pages 87–91, April 1999
How to Cite
Ranaldi, F., Vanni, P. and Giachetti, E. (1999), What students must know about the determination of enzyme kinetic parameters. Biochemical Education, 27: 87–91. doi: 10.1016/S0307-4412(98)00301-X
- Issue published online: 30 JUN 2010
- Article first published online: 30 JUN 2010
- Cited By
After a brief overview of the limits of the graphical methods used to determine enzyme kinetic parameters, the paper shows the results of their application to simulated velocity data, influenced by experimental errors of increasing magnitude. The comparison indicates that the best method to evaluate Vmax and Km is nonlinear regression, even in the presence of constant relative error; whereas the double reciprocal plot should be avoided, unless used with a proper weighting factor. The paper also suggests a simple method to obtain computer-simulated velocity data, with which the student may get direct practise and experience.