1574-6968/asset/FML_left.gif?v=1&s=d9ad90a5f75253894fe5059aa2f75bf910ebf83a)
1574-6968/asset/FML_right.gif?v=1&s=48d5e33deef512c09651020f71074ad93d3351e7)
Cloning and expression of a putative cyclodextrin glucosyltransferase from the symbiotically competent cyanobacterium Nostoc sp. PCC 9229
Article first published online: 9 JAN 2006
DOI: 10.1016/S0378-1097(02)01204-1
1574-6968/asset/cover.gif?v=1&s=069bb2c224e243333d4486580fbd90d9ebeffa6b "FEMS Microbiology Letters")
Additional Information
How to Cite
Wouters, J., Bergman, B. and Janson, S. (2003), Cloning and expression of a putative cyclodextrin glucosyltransferase from the symbiotically competent cyanobacterium Nostoc sp. PCC 9229. FEMS Microbiology Letters, 219: 181–185. doi: 10.1016/S0378-1097(02)01204-1
Publication History
- Issue published online: 9 JAN 2006
- Article first published online: 9 JAN 2006
- Received 2 October 2002, Revised 10 December 2002, Accepted 10 December 2002
- Abstract
- Article
- References
- Cited By
Keywords:
- cgt;
- CGTase;
- Differential display;
- Symbiosis;
- Nostoc;
- Gunnera
Abstract
A polymerase chain reaction-based method was used to isolate a Nostoc sp. PCC 9229 cDNA from infected glands of Gunnera chilensis. The complete gene sequence was isolated from a genomic Nostoc sp. PCC 9229 library. Sequence analysis showed 84% amino acid similarity to a putative cyclodextrin glycosyltransferase from Nostoc sp. PCC 7120 and the gene was therefore termed cgt. Southern blot revealed that the cgt gene was present in symbiotically competent cyanobacteria. The cgt gene was expressed in free-living nitrogen-fixing cultures in light or in darkness when supplemented with fructose. This is the first expression analysis of a cgt gene from a cyanobacterium.