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Cloning and expression of hyaluronate lyase genes of Streptococcus intermedius and Streptococcus constellatus subsp. constellatus†

  1. Ayuko Takao*

Article first published online: 9 JAN 2006

DOI: 10.1016/S0378-1097(03)00023-5

Issue

FEMS Microbiology Letters

FEMS Microbiology Letters

Volume 219, Issue 1, pages 143–150, February 2003

Additional Information

How to Cite

Takao, A. (2003), Cloning and expression of hyaluronate lyase genes of Streptococcus intermedius and Streptococcus constellatus subsp. constellatus. FEMS Microbiology Letters, 219: 143–150. doi: 10.1016/S0378-1097(03)00023-5

Author Information

  1. Department of Oral Bacteriology, School of Dental Medicine, Tsurumi University, 2–1–3 Tsurumi, Tsurumi-ku, Yokohama 230-8501 Japan

* *Tel.: +81 (45) 580 8441; Fax: +81 (45) 573 9599, E-mail address: takao-a@tsurumi-u.ac.jp

  • †

    All nucleotide sequence information in this paper was submitted to GenBank, and the accession numbers were obtained (AF385683, AF385684).

Publication History

  1. Issue published online: 9 JAN 2006
  2. Article first published online: 9 JAN 2006
  3. Received 30 August 2002, Revised 10 December 2002, Accepted 18 December 2002

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Keywords:

  • Hyaluronate lyase gene;
  • Zymography;
  • Streptococcus intermedius;
  • Streptococcus constellatus subsp. constellatus

Abstract

Hyaluronate lyase (HAase) genes of Streptococcus intermedius and Streptococcus constellatus subsp. constellatus were isolated. In S. constellatus subsp. constellatus, the deduced amino acid sequence of HAase was most similar to that of S. intermedius (68%), whereas the enzyme of S. intermedius was most similar to that of S. pneumoniae (72%). Upstream of the HAase gene on the opposite strands, an open reading frame of a putative glutathione peroxidase started in S. intermedius, and this arrangement was similar to that in S. pneumoniae but unlike that in S. constellatus subsp. constellatus. Cell lysates of Escherichia coli carrying each streptococcal gene showed HAase activity, demonstrating that each cloned gene actually coded for HAase.

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