You have full text access to this OnlineOpen article

An aromatic amino acid auxotrophic mutant of Bordetella bronchiseptica is attenuated and immunogenic in a mouse model of infection

  1. Jason D McArthur1,
  2. Nicholas P West1,
  3. Jason N Cole1,
  4. Heidrun Jungnitz2,
  5. Carlos A Guzmán2,
  6. James Chin3,
  7. Philip R Lehrbach4,
  8. Steven P Djordjevic3,
  9. Mark J Walker1,*

Article first published online: 9 JAN 2006

DOI: 10.1016/S0378-1097(03)00162-9

Issue

FEMS Microbiology Letters

FEMS Microbiology Letters

Volume 221, Issue 1, pages 7–16, April 2003

Additional Information

How to Cite

McArthur, J. D., West, N. P., Cole, J. N., Jungnitz, H., Guzmán, C. A., Chin, J., Lehrbach, P. R., Djordjevic, S. P. and Walker, M. J. (2003), An aromatic amino acid auxotrophic mutant of Bordetella bronchiseptica is attenuated and immunogenic in a mouse model of infection. FEMS Microbiology Letters, 221: 7–16. doi: 10.1016/S0378-1097(03)00162-9

Author Information

  1. 1

    Department of Biological Sciences, University of Wollongong, Wollongong, NSW 2522, Australia

  2. 2

    Vaccine Research Group, Division of Microbiology, GBF-German Research Centre for Biotechnology, Braunschweig, Germany

  3. 3

    NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Camden, NSW, Australia

  4. 4

    Fort Dodge Australia Pty. Ltd., Baulkham Hills, NSW, Australia

* *Corresponding author. Tel.: +61 (242) 213439; Fax: +61 (242) 214135, E-mail address: mwalker@uow.edu.au

Publication History

  1. Issue published online: 9 JAN 2006
  2. Article first published online: 9 JAN 2006
  3. Received 4 September 2002, Revised 13 January 2003, Accepted 15 January 2003

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (376K)

Keywords:

  • Respiratory infection;
  • Metabolic mutation;
  • Antibody

Abstract

We have constructed an aromatic amino acid auxotrophic mutant of Bordetella bronchiseptica, harbouring mutations in aroA and trpE to investigate the use of such a strain as a live-attenuated vaccine. B. bronchiseptica aroA trpE was unable to grow in minimal medium without aromatic supplementation. Compared to the parental wild-type strain, the mutant displayed significantly reduced abilities to invade and survive within the mouse macrophage-like cell line J774A.1 in vitro and in the murine respiratory tract following experimental intranasal infection. Mice vaccinated with B. bronchiseptica aroA trpE displayed significant dose-dependent increases in B. bronchiseptica-specific antibody responses, and exhibited increases in the number of B. bronchiseptica-reactive spleen cells in lymphoproliferation assays. Immunised animals were protected against lung colonisation after challenge with the wild-type parental strain. With such a broad host range displayed by B. bronchiseptica, the attenuated strain constructed in this study may not only be used for the prevention of B. bronchiseptica-associated disease, but also for the potential delivery of heterologous antigen.

View Full Article (HTML) Get PDF (376K)