Carbon supply and 2-oxoglutarate effects on expression of nitrate reductase and nitrogen-regulated genes in Synechococcus sp. strain PCC 7942

Authors

  • Marı́a Félix Vázquez-Bermúdez,

    1. Instituto de Bioquı́mica Vegetal y Fotosı́ntesis, CSIC-Universidad de Sevilla, Avda. Américo Vespucio s/n, E-41092 Seville, Spain
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  • Antonia Herrero,

    1. Instituto de Bioquı́mica Vegetal y Fotosı́ntesis, CSIC-Universidad de Sevilla, Avda. Américo Vespucio s/n, E-41092 Seville, Spain
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  • Enrique Flores

    Corresponding author
    1. Instituto de Bioquı́mica Vegetal y Fotosı́ntesis, CSIC-Universidad de Sevilla, Avda. Américo Vespucio s/n, E-41092 Seville, Spain
      *Corresponding author. Tel.: +34 (95) 448 9523; Fax: +34 (95) 446 0065, E-mail address: flores@cica.es
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*Corresponding author. Tel.: +34 (95) 448 9523; Fax: +34 (95) 446 0065, E-mail address: flores@cica.es

Abstract

Synthesis of nitrate reductase in the unicellular cyanobacterium Synechococcus sp. strain PCC 7942 took place at a slow rate when the cells were incubated without a supply of inorganic carbon, but addition to these cells of CO2/bicarbonate or, in a Synechococcus strain transformed with a gene encoding a 2-oxoglutarate permease, 2-oxoglutarate stimulated expression of the enzyme. Induction by 2-oxoglutarate was also observed at the mRNA level for two nitrogen-regulated genes, nir and amt1, but not for the photosystem II D1 protein-encoding gene psbA1. Our results are consistent with a role of 2-oxoglutarate in nitrogen control in cyanobacteria.

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