These authors contributed equally to the work.
Root exudate of pmi tomato mutant M161 reduces AM fungal proliferation in vitro
Article first published online: 9 JAN 2006
FEMS Microbiology Letters
Volume 223, Issue 2, pages 193–198, June 2003
How to Cite
Gadkar, V., David-Schwartz, R., Nagahashi, G., Douds, D. D., Wininger, S. and Kapulnik, Y. (2003), Root exudate of pmi tomato mutant M161 reduces AM fungal proliferation in vitro. FEMS Microbiology Letters, 223: 193–198. doi: 10.1016/S0378-1097(03)00357-4
- Issue published online: 9 JAN 2006
- Article first published online: 9 JAN 2006
- Received 26 March 2003, Revised 20 April 2003, Accepted 22 April 2003
- Root exudate;
- Mycorrhizal mutant;
- Glomus intraradices;
- Gigaspora gigantea;
- Gigaspora rosea
Soluble factors released from roots of the pre-mycorrhizal infection (pmi) myc− tomato mutant M161 were analyzed and compared with normal wild-type released factors. Aseptic whole exudates from the M161 mutant retarded the proliferation of Glomus intraradices in vitro. When the whole exudate was further fractionated on a C18 SEPAK cartridge, the 50/70% methanol fraction showed an activity against hyphal tip growth of Gigaspora gigantea and Gl. intraradices. Preliminary characterization of the exudate suggests that the inhibitory moieties are heat labile, bind to PVPP (polyvinyl polypyrrolidone), and are not volatile. This is the first reported instance of the inhibition by a myc− plant being ascribed to inhibitory component(s) released in root exudate.