Department of Molecular Biology, John Innes Centre, Colney, Norwich NR4 7UH, UK.
BldD from Streptomyces coelicolor is a non-essential global regulator that binds its own promoter as a dimer
Article first published online: 9 JAN 2006
FEMS Microbiology Letters
Volume 225, Issue 1, pages 35–40, August 2003
How to Cite
Elliot, M. A., Locke, T. R., Galibois, C. M. and Leskiw, B. K. (2003), BldD from Streptomyces coelicolor is a non-essential global regulator that binds its own promoter as a dimer. FEMS Microbiology Letters, 225: 35–40. doi: 10.1016/S0378-1097(03)00474-9
- Issue published online: 9 JAN 2006
- Article first published online: 9 JAN 2006
- Received 2 April 2003, Revised 21 May 2003, Accepted 4 June 2003
- Antibiotic production;
- Morphological differentiation;
- Global regulator;
- DNA binding
We have shown that the bldD gene of Streptomyces coelicolor, while required for antibiotic production and morphological differentiation, is not essential for viability. We have also demonstrated that BldD forms a higher order complex both in solution and when bound to target DNA. Purified BldD exists in three forms in solution, as a tetramer, dimer and monomer, but only in the dimeric form when bound to its own promoter/operator.