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Localizing cell division in spherical Escherichia coli by nucleoid occlusion

  1. Arieh Zaritsky1,*,
  2. Conrad L Woldringh2

Article first published online: 9 JAN 2006

DOI: 10.1016/S0378-1097(03)00580-9

Issue

FEMS Microbiology Letters

FEMS Microbiology Letters

Volume 226, Issue 2, pages 209–214, September 2003

Additional Information

How to Cite

Zaritsky, A. and Woldringh, C. L. (2003), Localizing cell division in spherical Escherichia coli by nucleoid occlusion. FEMS Microbiology Letters, 226: 209–214. doi: 10.1016/S0378-1097(03)00580-9

Author Information

  1. 1

    Department of Life Sciences, Ben-Gurion University of the Negev, P.O. Box 653, Be'er-Sheva 84105, Israel

  2. 2

    Swammerdam Institute for Life Sciences, BioCentrum, University of Amsterdam, Kruislaan 316, 1098 SM Amsterdam, The Netherlands

* *Corresponding author. Tel.: +972 (8) 6461 712; Fax: +972 (8) 6278 951, E-mail address: ariehz@bgumail.bgu.ac.il

Publication History

  1. Issue published online: 9 JAN 2006
  2. Article first published online: 9 JAN 2006
  3. Received 27 November 2002, Revised 13 February 2003, Accepted 1 March 2003

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Keywords:

  • FtsZ-GFP and DAPI fluorescence;
  • Bacterial nucleoid;
  • Spatial control of cell division

Abstract

The spatial relationship between FtsZ localization and nucleoid segregation was followed in Escherichia coli thyA cells, made spheroidal by brief exposure to mecillinam and after manipulating chromosome replication time using changes (‘steps’) in thymine concentration [Zaritsky et al., Microbiology 145 (1999) 1015–1022]. In such cells, fluorescent FtsZ-GFP arcs did not overlap the DAPI-stained nucleoids. It is concluded that FtsZ rings are deposited between segregating nucleoids, consistent with the nucleoid occlusion model [Woldringh et al., J. Bacteriol. 176 (1994) 6030–6038].

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