A double-stranded RNA mycovirus confers hypovirulence-associated traits to Botrytis cinerea

Authors

  • Miguel Castro,

    1. Laboratorio de Virologı́a de Hongos, Departamento de Biologı́a, Facultad de Quı́mica y Biologı́a, Universidad de Santiago de Chile, Casilla 40, Correo 33, Santiago, Chile
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  • Kurt Kramer,

    1. Laboratorio de Virologı́a de Hongos, Departamento de Biologı́a, Facultad de Quı́mica y Biologı́a, Universidad de Santiago de Chile, Casilla 40, Correo 33, Santiago, Chile
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  • Lorena Valdivia,

    1. Laboratorio de Virologı́a de Hongos, Departamento de Biologı́a, Facultad de Quı́mica y Biologı́a, Universidad de Santiago de Chile, Casilla 40, Correo 33, Santiago, Chile
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  • Sylvia Ortiz,

    1. Laboratorio de Virologı́a de Hongos, Departamento de Biologı́a, Facultad de Quı́mica y Biologı́a, Universidad de Santiago de Chile, Casilla 40, Correo 33, Santiago, Chile
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  • Antonio Castillo

    Corresponding author
    1. Laboratorio de Virologı́a de Hongos, Departamento de Biologı́a, Facultad de Quı́mica y Biologı́a, Universidad de Santiago de Chile, Casilla 40, Correo 33, Santiago, Chile
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*Corresponding author. Tel.: +56 (2) 681-2575; Fax: +56 (2) 681-2108, E-mail address: acastill@lauca.usach.cl

Abstract

Botrytis cinerea CCg425 contains a 33-nm isometric mycovirus whose genome is a 6.8-kb double-stranded RNA (dsRNA) molecule. Virulence bioassays, performed by direct plug mycelial inoculation on bean plant leaves, showed that B. cinerea CCg425 displays less fungal aggressivity than B. cinerea CKg54, a virulent fungal strain that is not infected by dsRNA mycoviruses. B. cinerea CCg425 also showed lower laccase activity and conidiation rate than B. cinerea CKg54. Furthermore, infection of B. cinerea CKg54 with viral particles purified from B. cinerea CCg425 resulted in diminished virulence of the infected fungus. Collectively, our results indicate that mycovirus infection confers hypovirulence to the fungal host.

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