• [1]
    Martin, F., Delaruelle, A., Hilberty, J.L. (1990) An improved ergosterol assay to estimate fungal biomass in ectomycorrhizas. Mycol. Res. 94, 10591064.
  • [2]
    Plassard, C.S., Mousain, D.G., Salsac, L.E. (1982) Estimation of mycelial growth of basidiomycetes by means of chitin determination. Phytochemistry 21, 345348.
  • [3]
    Balesdent, M.H., Desthieux, I., Gall, C., Robin, P., Rouxel, T. (1995) Quantification of Leptosphaeria-maculans growth in cotyledons of Brassica-napus using ELISA. J. Phytopathol. 143, 6573.
  • [4]
    Harrison, J.G., Barker, H., Lowe, R., Rees, E.A. (1990) Estimation of amounts of Phytophthora-infestans mycelium in leaf tissue by enzyme-linked-immunosorbent-assay. Plant Pathol. 39, 274277.
  • [5]
    Kamoun, S., van West, P., Govers, F. (1998) Quantification of late blight resistance of potato using transgenic Phytophthora infestans expressing beta-glucuronidase. Eur. J. Plant Pathol. 104, 521525.
  • [6]
    Oliver, R.P., Farman, M.L., Jones, J.D.G., Hammond-Kosack, K.E. (1993) Use of fungal transformants expressing beta-glucuronidase activity to detect infection and measure hyphal biomass in infected-plant tissues. Mol. Plant-Microbe Interact. 6, 521525.
  • [7]
    Thomma, B.P.H.J., Tadesse, Y.S.H., Jacobs, M., Broekaert, W.F. (1999) Disturbed correlation between fungal biomass and β-glucuronidase activity in infections of Arabidopsis thaliana with transgenic Alternaria brassicicola. Plant Sci. 148, 3136.
  • [8]
    Dewey, F.M. and Thornton, C.R. (1995) Fungal immunodiagnostics in plant agriculture. In: New Diagnostics in Crop Sciences (Skerrit, J.H. and Appels, R., Eds.), pp. 151–170. CAB Int., Cambridge University Press, Cambridge.
  • [9]
    Miller, S.A. (1996) Detecting propagules of plant pathogenic fungi. Adv. Bot. Res. 23, 73102.
  • [10]
    Mahe, A., Grisvard, J., Dron, M. (1992) Fungal-specific and plant-specific gene markers to follow the bean anthracnose infection process and normalize a bean chitinase mRNA induction. Mol. Plant-Microbe Interact. 5, 242248.
  • [11]
    Thomma, B.P.H.J., Eggermont, K., Penninckx, I.A.M.A., Mauch-Mani, B., Vogelsang, R., Cammue, B.P.A., Broekaert, W.F. (1998) Separate jasmonate-dependent and salicylate-dependent defense response pathways in Arabidopsis are essential for resistance to distinct microbial pathogens. Proc. Natl. Acad. Sci. USA 95, 1510715111.
  • [12]
    Hu, X., Nazar, R.N., Robb, J. (1993) Quantification of Verticillium biomass in wilt disease development. Phys. Mol. Plant Pathol. 42, 2336.
  • [13]
    Lamar, R.T., Schoenike, B., Vanden Wymelenberg, A., Stewart, P., Dietrich, D.M., Cullen, D. (1995) Quantitation of fungal mRNAs in complex substrates by reverse transcription PCR and its application to Phanerochaete chrysosporium-colonized soil. Appl. Environ. Microbiol. 61, 21222126.
  • [14]
    Heid, C.A., Stevens, J., Livak, K.J., Williams, P.M. (1996) Real time quantitative PCR. Genome Res. 6, 986994.
  • [15]
    Mackay, I.M., Arden, K.E., Nitsche, A. (2002) Real-time PCR in virology. Nucleic Acids Res. 30, 12921305.
  • [16]
    Wittwer, C.T., Ririe, K.M., Andrew, R.V., David, D.A., Gundry, R.A., Balis, U.J. (1997) The LightCycler™: a microvolume multisample fluorimeter with rapid temperature control. BioTechniques 22, 176181.
  • [17]
    Holland, P.M., Abramson, R.D., Watson, R., Gelfand, D.H. (1991) Detection of specific polymerase chain reaction product by utilizing the 5′–3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88, 72767280.
  • [18]
    Tyagi, S., Kramer, F.R. (1996) Molecular beacons: probes that fluoresce upon hybridization. Nat. Biotechnol. 14, 303308.
  • [19]
    Whitcombe, D., Theaker, J., Guy, S.P., Brown, T., Little, S. (1999) Detection of PCR products using self-probing amplicons and fluorescence. Nat. Biotechnol. 17, 804807.
  • [20]
    Morrison, T.B., Weis, J.J., Wittwer, C.T. (1998) Quantification of low-copy transcripts by continuous SYBR Green I monitoring during amplification. BioTechniques 24, 954962.
  • [21]
    Ausubel, F.M., Katagiri, F., Mindrinos, M., Glazebrook, J. (1995) Use of Arabidopsis thaliana defense-related mutants to dissect the plant response to pathogens. Proc. Natl. Acad. Sci. USA 92, 41894196.
  • [22]
    Thomma, B.P.H.J., Penninckx, I.A.M.A., Broekaert, W.F., Cammue, B.P.A. (2001) The complexity of disease signaling in Arabidopsis. Curr. Opin. Immunol. 13, 6368.
  • [23]
    Bent, A.F., Innes, R.W., Ecker, J.R., Staskawicz, B.J. (1992) Disease development in ethylene-insensitive Arabidopsis thaliana infected with virulent and avirulent Pseudomonas and Xanthomonas pathogens. Mol. Plant-Microbe Interact. 5, 372378.
  • [24]
    Hoffman, T., Schmidt, J.S., Zheng, X., Bent, A.F. (1999) Isolation of ethylene-insensitive soybean mutants that are altered in pathogen susceptibility and gene-for-gene disease resistance. Plant Physiol. 119, 935950.
  • [25]
    Thomma, B.P.H.J., Eggermont, K., Tierens, K.F.M.-J., Broekaert, W.F. (1999) Requirement of functional EIN2 (ethylene insensitive 2) gene for efficient resistance of Arabidopsis thaliana to infection by Botrytis cinerea. Plant Physiol. 121, 10931101.
  • [26]
    Schaad, N.W., Frederick, R.D. (2002) Real-time PCR and its application for rapid plant disease diagnostics. Can. J. Plant Pathol. 24, 250258.
  • [27]
    Parker, J.E., Holub, E.B., Frost, L.N., Falk, A., Gunn, N.D., Daniels, M.J. (1996) Characterization of eds1, a mutation in Arabidopsis suppressing resistance to Peronospora parasitica specified by several different RPP genes. Plant Cell 8, 20332046.
  • [28]
    Thomma, B.P.H.J., Nelissen, I., Eggermont, K., Broekaert, W.F. (1999) Deficiency in phytoalexin production causes enhanced susceptibility of Arabidopsis thaliana to the fungus Alternaria brassicicola. Plant J. 19, 163171.
  • [29]
    The Arabidopsis Genome Initiative (2000) Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408, 796–815.
  • [30]
    Glazebrook, J. (2001) Genes controlling expression of defense responses in Arabidopsis-2001 status. Curr. Opin. Plant Biol. 4, 301308.
  • [31]
    Böhm, J., Hahn, A., Schubert, R., Bahnweg, G., Adler, N., Nechwatal, J., Oehlmann, R., Osswald, W. (1999) Real-time quantitative PCR: DNA determination in isolated spores of the mycorrhizal fungus Glomus mosseae and monitoring of Phytophora infestans and Phytophthora citricola in their respective host plants. Phytopathol. Z. 14, 409416.
  • [32]
    Schaad, N.W., Berthier-Schaad, Y., Sechler, A., Knorr, D. (1999) Detection of Clavibacter michiganensis subsp. sepedonicus in potato tubers by BIO-PCR and an automated real-time fluorescence detection system. Plant Dis. 83, 10951100.
  • [33]
    Weller, A., Elphinstone, J.G., Smith, N.C., Boonham, N., Stead, D.E. (2000) Detection of Ralstonia solanacearum strains with a quantitative, multiplex, real-time, fluorogenic PCR (TaqMan) assay. Appl. Environ. Microbiol. 66, 28532858.
  • [34]
    Winton, L.M., Stone, J.K., Watrud, L.S., Hansen, E.M. (2002) Simultaneous one-tube quantification of host and pathogen DNA with real-time polymerase chain reaction. Phytopathology 92, 112116.