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Keywords:

  • Culturable canine intestinal lactic acid bacteria;
  • Jejunal chyme;
  • Streptococcus alactolyticus

Abstract

Canine intestinal lactic acid bacterium (LAB) population in four fistulated dogs was cultured and enumerated using MRS agar. LAB levels ranging from 1.4×106 to 1.5×107 CFU ml−1 were obtained in jejunal chyme. In the fecal samples 7.0×107 and 2.0×108 CFU g−1 were detected. Thirty randomly selected isolates growing in the highest sample dilutions were identified to species level using numerical analysis of 16S and 23S rDNA restriction fragment length polymorphism patterns (ribotyping) and 16S rDNA sequence analysis. According to these results, Streptococcus alactolyticus was the dominant culturable LAB species in both feces and jejunal chyme. In addition, Lactobacillus murinus and Lactobacillus reuteri were detected.