l-Arginine and mitomycin C-induced nitric oxide release and apoptosis in human lymphocytes
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 2003 International Federation for Cell Biology
Cell Biology International
Volume 27, Issue 4, pages 337–340, April 2003
How to Cite
Erden, C. D., Ekmekci, A., Şahin, F. I., Ergün, M. A., Öztürk, G. and Erbas, D. (2003), l-Arginine and mitomycin C-induced nitric oxide release and apoptosis in human lymphocytes. Cell Biology International, 27: 337–340. doi: 10.1016/S1065-6995(02)00350-5
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Received 18 February 2002, revised 6 October 2002, accepted 14 November 2002
- Nitric oxide;
- Mitomycin C;
Nitric oxide (NO) is a free radical that is produced by a number of mammalian cell types from l-arginine and a critical mediator that acts in many tissues to regulate a diverse range of physiological processes. The major metabolic end product for NO is nitrate (NO3) and nitrite (NO2), which are stable metabolites within tissue, plasma, and urine. Measurements of nitrate and nitrite values reveal alterations in NO production. Endogenously generated or exogenously applied NO causes DNA cleavage by endonuclease activation.
We investigated the effect of l-arginine and mitomycin C (MMC) on cultured lymphocytes of healthy individuals. We observed chromosome breaks, apoptotic cells and increased NO levels after l-arginine and MMC addition. In conclusion, our results confirmed that NO may be the cause of apoptotic cell death in l-arginine added lymphocyte culture.