Flow cytometric cell-cycle analysis of cultured fibroblasts from the giant panda, Ailuropoda melanoleuca L.

Authors

  • Zhi-Ming Han,

    1. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China
    2. The College of Life Sciences, Beijing Normal University, Beijing 100875, People's Republic of China
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  • Da-Yuan Chen,

    Corresponding author
    1. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China
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  • Jin-Song Li,

    1. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China
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  • Qing-Yuan Sun,

    1. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China
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  • Peng-Yan Wang,

    1. China Research and Conservation Center for Giant Panda, Wolong Nature Reserve, Sichuan 623006, People's Republic of China
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  • Jun Du,

    1. China Research and Conservation Center for Giant Panda, Wolong Nature Reserve, Sichuan 623006, People's Republic of China
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  • He-Min Zhang

    1. China Research and Conservation Center for Giant Panda, Wolong Nature Reserve, Sichuan 623006, People's Republic of China
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Corresponding author. Tel.: +86-10-62560528/62793; fax: +86-10-62565689. chendy@panda.ioz.ac.cn

Abstract

In animal cloning, it is generally believed that the inactive diploid G0or G1stage of the cell cycle is beneficial to initiate cell-cycle coordination and reprogramming following transfer of the donor nucleus. Previous experiments have demonstrated that serum starvation results in quiescent cell stage. Some experiments show that the majority of cells in a fully confluent cell culture are also in an inactive G1stage.

In order to provide more G0/G1stage cells for giant panda cloning, we carried out a flow cytometric analysis of the cell cycle of fibroblasts from the abdominal muscle of a giant panda at different passage numbers under different growth conditions, and after different periods of serum starvation. The percentage of G0+G1stage cells differed significantly under different growth conditions. Serum starvation effectively increased the percentage of G0+G1stage cells, and the cell cycle characteristics following serum starvation for varying periods of time differed with this and the initial confluency of the cultures. The data should help in choosing the optimal stage for preparing donor cells as well as increasing the potential cloning efficiency in our study of giant panda cloning.

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