Spatio-temporal expression of Drosophila mitochondrial transcription factor A during development

Authors

  • Kei-ichi Takata,

    1. Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, Chiba-ken 278-8510, Japan
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  • Yoshihiro H. Inoue,

    1. Drosophila Genetic Resource Center, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan
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  • Fumiko Hirose,

    1. Division of Biochemistry, Aichi Cancer Center Research Institute, Nagoya, Aichi 464-8681, Japan
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  • Shizuka Murakami,

    1. Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, Chiba-ken 278-8510, Japan
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  • Kaori Shimanouchi,

    1. Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, Chiba-ken 278-8510, Japan
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  • Ippei Sakimoto,

    1. Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, Chiba-ken 278-8510, Japan
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  • Kengo Sakaguchi

    Corresponding author
    1. Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi, Chiba-ken 278-8510, Japan
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Corresponding author. Tel: +81-471-24-1501x3409;fax: +81-471-23-9767. kengo@rs.noda.tus.ac.jp

Abstract

We previously reported cloning of the Drosophila mitochondrial transcription factor A (D-mtTFA) gene, and characterization of a recombinant protein. In this report, the expression and distribution patterns of D-mtTFA during development are described. D-mtTFA mRNA and its protein product were found to be abundant in cells of tissues undergoing both proliferation and polytenization. Furthermore, a DRE-like sequence present in the D-mtTFA gene showed promotor activity in Drosophila Kc cells. In addition, D-mtTFA was detected in brain throughout the developmental process, as well as in non-proliferating tissues, such as flight muscle and cardia, and was also found in spermatids of imagos. D-mtTFA possesses a nuclear-targeting sequence, and is present in the nucleus at the syncytial stage, where bundles of 64 spermatids are present during spermatogenesis. The results suggest that D-mtTFA not only contributes to the mitochondrial DNA transcription and replication system, but also has a role in cell proliferation and development, including spermatogenesis.

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