Intranuclear Ca2+signals within individual nuclear lobes of neutrophils

Authors

  • Robert Hodges,

    1. Molecular Signalling Group, University Department of Surgery, University of Wales College of Medicine, Heath Park, CF14 4XN, Cardiff, UK
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  • Iraj Laffafian,

    1. Molecular Signalling Group, University Department of Surgery, University of Wales College of Medicine, Heath Park, CF14 4XN, Cardiff, UK
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  • Maurice B. Hallett

    Corresponding author
    1. Molecular Signalling Group, University Department of Surgery, University of Wales College of Medicine, Heath Park, CF14 4XN, Cardiff, UK
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Corresponding author. Tel.: +44-1222-742-748; fax: +44-1222-761-623 HallettMB@Cardiff.ac.uk

Abstract

Cytosolic free Ca2+and intranuclear Ca2+behave similarly in human neutrophils. However, conventional laser scanning at 350 ms/frame resolution at lower than physiological temperatures demonstrates that (i) the nuclear fluo3-Ca2+signal persists longer than the cytosolic signal in some (but not all) nuclear lobes, (ii) the neutrophil nuclear membrane and fine inter-lobe filaments present barriers to diffusion of fluo3-Ca2+and lucifer yellow, and (iii) the diffusion barrier correlates with condensed chromosomal material on the nuclear envelope and blockage of the movement of fluo3-Ca2+into individual nuclear lobes.

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