The expression of matrix metalloproteinase-13 and osteocalcin in mouse osteoblasts is related to osteoblastic differentiation and is modulated by 1,25-dihydroxyvitamin D3 and thyroid hormones

Authors

  • Nadja Fratzl-Zelman,

    Corresponding author
    1. Ludwig Boltzmann Institute of Osteology, 4th Medical Department, Hanusch Hospital, Heinrich Collin-Str. 30, A-1140 Vienna, Austria
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    • Both authors contributed equally to this work.

  • Helmut Glantschnig,

    1. Ludwig Boltzmann Institute of Osteology, 4th Medical Department, Hanusch Hospital, Heinrich Collin-Str. 30, A-1140 Vienna, Austria
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    • Both authors contributed equally to this work.

  • Monika Rumpler,

    1. Ludwig Boltzmann Institute of Osteology, 4th Medical Department, Hanusch Hospital, Heinrich Collin-Str. 30, A-1140 Vienna, Austria
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  • Alexander Nader,

    1. Ludwig Boltzmann Institute of Osteology, 4th Medical Department, Hanusch Hospital, Heinrich Collin-Str. 30, A-1140 Vienna, Austria
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  • Adolf Ellinger,

    1. Ludwig Boltzmann Institute of Osteology, 4th Medical Department, Hanusch Hospital, Heinrich Collin-Str. 30, A-1140 Vienna, Austria
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  • Franz Varga

    1. Ludwig Boltzmann Institute of Osteology, 4th Medical Department, Hanusch Hospital, Heinrich Collin-Str. 30, A-1140 Vienna, Austria
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Corresponding author. Tel.: +43-1-91021-86921; fax: +43-1-91021-86929 fratzl@ap.univie.ac.at

Abstract

Matrix metalloproteinase-13 (MMP-13), is a key protein of bone matrix degradation, and is highly expressed by osteoblasts. We used the osteoblast-like MC3T3-E1 cell line and compared the stimulatory effects of the bone resorptive agents 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) 3,3′,5-triido-l-thyronine (T3) on the expression of MMP-13 mRNA. We showed that the stimulatory effects were time and dose dependent, and were also transduced to the protein level, with 1,25-(OH)2D3being more potent.

MMP-13 expression in different mouse cells and its localization within developing bone from the onset of osteogenesis were also investigated. 1,25-(OH)2D3- and T3-regulated osteocalcin (Osc) expression in mouse osteoblasts was compared to hormonal effects on MMP-13 expression and activity. Here we show divergent and common roles of 1,25-(OH)2D3and T3 action on the expression of these marker proteins, depending on the stage of cell differentiation. In addition, we propose a role for MMP-13 in the bone collar of developing long bones. The results could help to more precisely characterize hormonal regulation in the developmental sequence of osteoblasts.

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