Both authors contributed equally to this work.
The expression of matrix metalloproteinase-13 and osteocalcin in mouse osteoblasts is related to osteoblastic differentiation and is modulated by 1,25-dihydroxyvitamin D3 and thyroid hormones
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 2003 International Federation for Cell Biology
Cell Biology International
Volume 27, Issue 6, pages 459–468, June 2003
How to Cite
Fratzl-Zelman, N., Glantschnig, H., Rumpler, M., Nader, A., Ellinger, A. and Varga, F. (2003), The expression of matrix metalloproteinase-13 and osteocalcin in mouse osteoblasts is related to osteoblastic differentiation and is modulated by 1,25-dihydroxyvitamin D3 and thyroid hormones. Cell Biology International, 27: 459–468. doi: 10.1016/S1065-6995(03)00037-4
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Received 28 May 2002, revised 16 December 2002, accepted 12 February 2003
Matrix metalloproteinase-13 (MMP-13), is a key protein of bone matrix degradation, and is highly expressed by osteoblasts. We used the osteoblast-like MC3T3-E1 cell line and compared the stimulatory effects of the bone resorptive agents 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) 3,3′,5-triido-l-thyronine (T3) on the expression of MMP-13 mRNA. We showed that the stimulatory effects were time and dose dependent, and were also transduced to the protein level, with 1,25-(OH)2D3being more potent.
MMP-13 expression in different mouse cells and its localization within developing bone from the onset of osteogenesis were also investigated. 1,25-(OH)2D3- and T3-regulated osteocalcin (Osc) expression in mouse osteoblasts was compared to hormonal effects on MMP-13 expression and activity. Here we show divergent and common roles of 1,25-(OH)2D3and T3 action on the expression of these marker proteins, depending on the stage of cell differentiation. In addition, we propose a role for MMP-13 in the bone collar of developing long bones. The results could help to more precisely characterize hormonal regulation in the developmental sequence of osteoblasts.