Actin expression in Taenia solium cysticerci (cestoda): tisular distribution and detection of isoforms

Authors

  • Javier R. Ambrosio,

    Corresponding author
    1. Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Edificio A, 2o Piso, Circ. Int. Cd. Universitaria, Mexico D.F. 04510, Mexico
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  • Olivia Reynoso-Ducoing,

    1. Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Edificio A, 2o Piso, Circ. Int. Cd. Universitaria, Mexico D.F. 04510, Mexico
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  • Hugo Hernández-Sanchez,

    1. Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Edificio A, 2o Piso, Circ. Int. Cd. Universitaria, Mexico D.F. 04510, Mexico
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  • David Correa-Piña,

    1. Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Edificio A, 2o Piso, Circ. Int. Cd. Universitaria, Mexico D.F. 04510, Mexico
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  • Laura González-Malerva,

    1. Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Edificio A, 2o Piso, Circ. Int. Cd. Universitaria, Mexico D.F. 04510, Mexico
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  • Mayra Cruz-Rivera,

    1. Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Edificio A, 2o Piso, Circ. Int. Cd. Universitaria, Mexico D.F. 04510, Mexico
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  • Ana Flisser

    1. Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Edificio A, 2o Piso, Circ. Int. Cd. Universitaria, Mexico D.F. 04510, Mexico
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Corresponding author. Tel.: +52-55-5623-2467;fax: +52-55-5623-2382 jrah@servidor.unam.mx

Abstract

Identification, localization and partial biochemical characterization of actins expressed in the larval stage of the cestode parasite Taenia solium has been carried out. Frozen tissue sections of cysticerci, the larval stage of this parasite, were reacted with rhodamine-phalloidin, parasite actin was purified by polymerization in the presence of K+, mg++and ATP actin was analyzed by SDS—PAGE and two-dimensional gel electrophoresis, and immunoblotting of actin was performed in PVDF membranes and with commercial anti-actin monoclonal antibodies. Parasitic tissues showed different fibrous actin fluorescence patterns, which correlated with the expression of isoactins. Purified globular actin had a similar molecular mass to rabbit commercial actin (∼44 kDa). Actin was resolved into seven isoforms, indicating a family of actin genes.

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