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Ty1 insertions in intergenic regions of the genome of Saccharomyces cerevisiae transcribed by RNA polymerase III have no detectable selective effect

  1. Victoria M. Blanc†,
  2. Julian Adams*

Article first published online: 9 JAN 2006

DOI: 10.1016/S1567-1356(03)00199-5

Issue

FEMS Yeast Research

FEMS Yeast Research

Volume 4, Issue 4-5, pages 487–491, January 2004

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How to Cite

Blanc, V. M. and Adams, J. (2004), Ty1 insertions in intergenic regions of the genome of Saccharomyces cerevisiae transcribed by RNA polymerase III have no detectable selective effect. FEMS Yeast Research, 4: 487–491. doi: 10.1016/S1567-1356(03)00199-5

Author Information

  1. Department of Biology, University of Michigan, Ann Arbor, MI 48109-1048, USA

  1. †

    1Asterand Inc., Detroit, MI 48201, USA.

* *Corresponding author. Present address: Departments of Molecular Cellular and Developmental Biology, and Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI 48019-1048, USA. Tel.: +1 (734) 763 3431; Fax: +1 (734) 647 0884, E-mail address: julian@umich.edu

  1. †

    1Asterand Inc., Detroit, MI 48201, USA.

Publication History

  1. Issue published online: 9 JAN 2006
  2. Article first published online: 9 JAN 2006
  3. Received 10 July 2003, Revised 1 September 2003, Accepted 3 September 2003

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Keywords:

  • Evolution;
  • Continuous culture;
  • Far3

Abstract

The retrotransposon Ty1 of Saccharomyces cerevisiae inserts preferentially into intergenic regions in the vicinity of RNA polymerase III-transcribed genes. It has been suggested that this preference has evolved to minimize the deleterious effects of element transposition on the host genome, and thus to favor their evolutionary survival. This presupposes that such insertions have no selective effect. However, there has been no direct test of this hypothesis. Here we construct a series of strains containing single Ty1 insertions in the vicinity of tRNA genes, or in the rDNA cluster on chromosome XII, which are otherwise isogenic to strain 337, containing zero Ty1 elements. Competition experiments between 337 and the strains containing single Ty1 insertions revealed that in all cases, the Ty1 insertions have no selective effect in rich medium. These results are thus consistent with the hypothesis that the insertion site preference of Ty1 elements has evolved to minimize the deleterious effects of transposition on the host genome.

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