Expression of cell adhesion molecules in the adriamycin-induced esophageal atresia rat model

Authors

  • Melih Tugay,

    Corresponding author
    1. Faculty of Medicine, Departments of Pediatric Surgery, Histology, Embryology & Anatomy, Kocaeli University, Suadiye PTT Md. PK: 20, 34741, Istanbul, Turkey
      Corresponding author. Fax: +90-212-210-5037 tugaym@hotmail.com
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  • Serdar Filiz,

    1. Faculty of Medicine, Departments of Pediatric Surgery, Histology, Embryology & Anatomy, Kocaeli University, Suadiye PTT Md. PK: 20, 34741, Istanbul, Turkey
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  • Hakki Dalçik,

    1. Faculty of Medicine, Departments of Pediatric Surgery, Histology, Embryology & Anatomy, Kocaeli University, Suadiye PTT Md. PK: 20, 34741, Istanbul, Turkey
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  • B. Haluk Güvenç,

    1. Faculty of Medicine, Departments of Pediatric Surgery, Histology, Embryology & Anatomy, Kocaeli University, Suadiye PTT Md. PK: 20, 34741, Istanbul, Turkey
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  • Cannur Dalçik,

    1. Faculty of Medicine, Departments of Pediatric Surgery, Histology, Embryology & Anatomy, Kocaeli University, Suadiye PTT Md. PK: 20, 34741, Istanbul, Turkey
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  • Mevlit Korkmaz,

    1. Faculty of Medicine, Departments of Pediatric Surgery, Histology, Embryology & Anatomy, Kocaeli University, Suadiye PTT Md. PK: 20, 34741, Istanbul, Turkey
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  • Selami Sozubir

    1. Faculty of Medicine, Departments of Pediatric Surgery, Histology, Embryology & Anatomy, Kocaeli University, Suadiye PTT Md. PK: 20, 34741, Istanbul, Turkey
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Corresponding author. Fax: +90-212-210-5037 tugaym@hotmail.com

Abstract

Cell adhesion molecules are well-known membrane glycoproteins widely expressed during embryonic development that play a crucial role in cell division, migration and differentiation. We investigated the cell—matrix relationship using N-CAM and pan-cadherin adhesion molecules in the adriamycin-induced esophageal atresia (EA) rat model in the hope of finding a clue to the mechanisms of this unique anomaly.

Time-mated pregnant Sprague—Dawley rats were given either saline or adriamycin on days 8 and 9 of gestation. Embryos were harvested on the 18th day of gestation. Esophageal specimens obtained from adriamycin-exposed embryos with (EA+) or without esophageal atresia (EA−) and from saline-exposed embryos were immunostained with N-CAM and pan-cadherin primary antisera.

The esophageal specimens from control and EA− groups revealed similar immunostaining properties: weak N-CAM and pan-cadherin immunoreactivity. In contrast, the EA+ group showed intense immunoreactivity.

Our study demonstrated an increased synthesis of N-CAM and pan-cadherin in the epithelial cells of the atretic esophagus and trachea. These results suggest that embryonic cell—cell and cell—matrix interactions may play a crucial role in the development of adriamycin-induced EA.

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