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Keywords:

  • Selective inhibition;
  • 5-Fluorouracil;
  • Adriamycin;
  • Diterpene;
  • Isolated embryonic cell;
  • Xenopus embryo

Abstract

We used Xenopus embryo cells with a cell cycle of 20–30 min to detect inhibitory effects on cell proliferation. Inhibition of proliferation was observed when isolated embryonic cells were incubated for 16 h in a simple salt solution containing the well-known anticancer drugs 5-fluorouracil and adriamycin. In addition, three diterpene compounds isolated from the anticancer herbal medicine kansui: kansuinin B, 20-OD-ingenol Z, and 20-OD-ingenol E specifically inhibited the proliferation of isolated embryonic cells. The inhibitory compounds selected using the embryonic cells also inhibited proliferation of certain mammalian cell types.