Differentiation-dependent localisation of tissue-type plasminogen activator in human bladder urothelium
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 2004 International Federation for Cell Biology
Cell Biology International
Volume 28, Issue 5, pages 381–386, May 2004
How to Cite
Makuc, J., Mašera, A., Tršinar, B. and Jezernik, K. (2004), Differentiation-dependent localisation of tissue-type plasminogen activator in human bladder urothelium. Cell Biology International, 28: 381–386. doi: 10.1016/j.cellbi.2004.03.006
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Received 21 October 2003, revised 16 February 2004, accepted 15 March 2004
- Tissue-type plasminogen activator;
- Papillary tumours;
Human bladder urothelium is able to secrete tissue-type plasminogen activator (tPA). The aim of our study was to analyse localisation of tPA antigen in comparison to differentiation state of cells in samples of histologically normal urothelium and non-invasive tumours of the human urinary bladder. Twenty-five samples of normal urothelium and 31 non-invasive papillary tumours from 36 patients were examined. The presence of tPA antigen was evaluated immunohistochemically. Differentiation of superficial cells was assessed by the presence of urothelial cell differentiation markers, uroplakins (UPs; immunohistochemistry) and cell's apical surface architecture (scanning electron microscopy). All tissue samples stained anti-tPA positive. In normal urothelium, the intensity of anti-tPA staining was the strongest in superficial cells, which were well-differentiated. In tumours, all cell layers stained anti-tPA positive. The intensity of anti-tPA positive reaction in the upper cell layer correlated with the percentage of anti-UP positive superficial cells. Superficial cells showed various differentiation states.
The localisation of tPA antigen in human in vivo tissue is not confined to the well-differentiated superficial cells. Our results suggest a positive correlation between tPA secretion and cell differentiation.