Enrichment and characterization of mouse putative epidermal stem cells

Authors

  • Jia-xi Zhou,

    1. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 25 Beisihuanxilu, Haidian Beijing 100080, People's Republic of China
    2. Graduate School of the Chinese Academy of Sciences, 19 Yu-quan Road, Beijing 100039, People's Republic of China
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  • Li-wei Jia,

    1. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 25 Beisihuanxilu, Haidian Beijing 100080, People's Republic of China
    2. Graduate School of the Chinese Academy of Sciences, 19 Yu-quan Road, Beijing 100039, People's Republic of China
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  • Yong-jun Yang,

    1. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 25 Beisihuanxilu, Haidian Beijing 100080, People's Republic of China
    2. Graduate School of the Chinese Academy of Sciences, 19 Yu-quan Road, Beijing 100039, People's Republic of China
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  • Sha Peng,

    1. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 25 Beisihuanxilu, Haidian Beijing 100080, People's Republic of China
    2. Graduate School of the Chinese Academy of Sciences, 19 Yu-quan Road, Beijing 100039, People's Republic of China
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  • Yu-jing Cao,

    1. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 25 Beisihuanxilu, Haidian Beijing 100080, People's Republic of China
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  • En-kui Duan

    Corresponding author
    1. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 25 Beisihuanxilu, Haidian Beijing 100080, People's Republic of China
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Corresponding author. Tel.: +86-10-625-58112; fax: +86-10-925-29248. duane@ioz.ac.cn

Abstract

Epidermis, a continuously renewing tissue, is maintained by stem cells that proliferate and replenish worn out or damaged cells in the tissue during life. Cultured epidermal stem cells have great potential in scientific research and clinical application. However, isolating a pure and viable population of epidermal stem cells and culturing them has been challenging. In this study, putative epidermal stem cells of mouse were isolated by combining Hoechst 33342 and propidium iodide staining with fluorescence-activated cell sorting. Molecular markers expression pattern analysis showed that cytokeratin 14, integrin β1 and p63 are expressed in the sorted putative stem cells, but not active β-catenin, nestin and involucrin. Our results provide further supporting data that mouse putative epidermal stem cells could be successfully isolated by combining Hoechst dye staining with fluorescence-activated cell sorting and cultured in vitro. The cultured mouse putative epidermal stem cells could be used as a potent tool for studying stem cell biology and testing stem cell therapy.

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