Catalases protect cellular proteins from oxidative modification in Saccharomyces cerevisiae

Authors

  • Volodymyr I. Lushchak,

    Corresponding author
    1. Department of Biochemistry, Vassyl Stefanyk Precarpathian National University, 57 Shevchenko Str., 76025, Ivano-Frankivsk, Ukraine
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  • Dmytro V. Gospodaryov

    1. Department of Biochemistry, Vassyl Stefanyk Precarpathian National University, 57 Shevchenko Str., 76025, Ivano-Frankivsk, Ukraine
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Corresponding author. Fax: +380 3422 31574. lushchak@pu.if.ua

Abstract

The yeast Saccharomyces cerevisiae cells had higher antioxidant enzyme activities under growth in ethanol than that in glucose as a carbon and energy source. The correlations between catalase activity and protein carbonyl level (r 2 = 0.857), between catalase and glucose-6-phosphate dehydrogenase activities (r 2 = 0.924) and between protein carbonyl levels and glucose-6-phosphate dehydrogenase activity (r 2 = 0.988) under growth in ethanol were found. Growing in ethanol the strain deficient in cytosolic and peroxisomal catalases had 7.1-fold higher level of carbonyl proteins than that of wild-type strain. Our data suggest that in vivo catalases may protect glucose-6-phosphate dehydrogenase against oxidative inactivation.

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