Cellular organisation and differentiation of organelles in pre-meiotic rice anthers

Authors

  • Ezaz Al Mamun,

    Corresponding author
    1. Faculty of Agriculture, Food and Natural Resources, The University of Sydney, Sydney, NSW-2006, Australia
    2. Cooperative Research Centre for Sustainable Rice Production, c/- New South Wales Agriculture, Private Mail Bag, Yanco NSW 2703, Australia
    Search for more papers by this author
  • Laurence C. Cantrill,

    1. School of Biological Sciences, The University of Sydney, Sydney, NSW-2006, Australia
    2. Cooperative Research Centre for Sustainable Rice Production, c/- New South Wales Agriculture, Private Mail Bag, Yanco NSW 2703, Australia
    Search for more papers by this author
  • Robyn L. Overall,

    1. School of Biological Sciences, The University of Sydney, Sydney, NSW-2006, Australia
    2. Cooperative Research Centre for Sustainable Rice Production, c/- New South Wales Agriculture, Private Mail Bag, Yanco NSW 2703, Australia
    Search for more papers by this author
  • Bruce G. Sutton

    1. Faculty of Agriculture, Food and Natural Resources, The University of Sydney, Sydney, NSW-2006, Australia
    2. Cooperative Research Centre for Sustainable Rice Production, c/- New South Wales Agriculture, Private Mail Bag, Yanco NSW 2703, Australia
    Search for more papers by this author

Corresponding author. Faculty of Agriculture, Food and Natural Resources, John Woolley Building A20, The University of Sydney, New South Wales 2006, Australia. Tel.: +61 2 93512939; fax: +61 2 93514172. mamune@agric.usyd.edu.au

Abstract

Pre-meiotic cellular organisation of rice anthers has a great significance in pollen formation. We have used a combination of confocal laser and transmission electron microscopy (TEM) to characterise and differentiate organelles in pre-meiotic rice anthers. Along with the characteristic organelles in the cytoplasm the epidermal cells of the pre-meiotic rice anther are coated on their outer surface by a conspicuous bi-lamellate cuticle. Chloroplasts of the endothecium contain immature grana, thylakoids and also starch granules. These plastids clearly contain photosynthetic pigments as shown by autofluorescence in confocal microscope studies. Both confocal and TEM studies reveal clusters of mitochondria in the middle layer. The tapetum contains electron opaque ribosomes, bundles of mitochondria and plastids. The nuclei of the tapetum occupy a large volume of the cytoplasm indicating the onset of mitotic prophase. Intense Rhodamine 123 staining reveals that a major portion of the structurally indistinguishable organelles that were seen throughout the densely ribosomic cytoplasm of sporogenous cells are mitochondria.

Ancillary