Disruption and de novo formation of nanotubular membrane extensions in SW620 colon carcinoma cell line during cell division

Authors

  • Mauro A.A. Castro,

    1. Instituto de Física, Universidade Federal do Rio Grande do Sul, Avenue Bento Gonçalves 9500, C.P. 15051, Porto Alegre 91501-970, Brazil
    2. Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos 2600—anexo, Porto Alegre 90035-003, Brazil
    3. Universidade Luterana do Brasil, Rua Miguel Tostes 101, Canoas 92420-280, Brazil
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  • Verônica A. Grieneisen,

    1. Instituto de Física, Universidade Federal do Rio Grande do Sul, Avenue Bento Gonçalves 9500, C.P. 15051, Porto Alegre 91501-970, Brazil
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  • Rita M.C. De Almeida

    Corresponding author
    1. Instituto de Física, Universidade Federal do Rio Grande do Sul, Avenue Bento Gonçalves 9500, C.P. 15051, Porto Alegre 91501-970, Brazil
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Corresponding author. rita@if.ufrgs.br

Abstract

A novel biological principle of cell-to-cell interaction based on membrane continuity of nanotubular channels has recently been described. These contacts are extremely dynamic and sensitive to mechanical stress, which causes their rapid breakage and retraction. Here we demonstrate that functional mechanical stress generated during cell division can disrupt membrane nanotubes, which are formed de novo when filopodia-like projections on one cell make contact with a neighbouring cell, using the SW620 colon carcinoma cell line. Considering the general principal of decreasing cell—cell interactions during tumour progression, our observation is appealing because this new phenomenon may be valid for neoplastic cells.

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