Acute-phase related binding ability of p53 for the hormone response element of the haptoglobin gene in adult rats

Authors

  • M. Mihailović,

    1. Department of Molecular Biology, Institute for Biological Research, Despot Stephen Boulevard 142, 11060 Belgrade, Serbia and Montenegro
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  • S. Dinić,

    1. Department of Molecular Biology, Institute for Biological Research, Despot Stephen Boulevard 142, 11060 Belgrade, Serbia and Montenegro
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  • A. Uskoković,

    1. Department of Molecular Biology, Institute for Biological Research, Despot Stephen Boulevard 142, 11060 Belgrade, Serbia and Montenegro
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  • M. Petrović,

    1. Department of Molecular Biology, Institute for Biological Research, Despot Stephen Boulevard 142, 11060 Belgrade, Serbia and Montenegro
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  • I. Grigorov,

    1. Department of Molecular Biology, Institute for Biological Research, Despot Stephen Boulevard 142, 11060 Belgrade, Serbia and Montenegro
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  • G. Poznanović,

    1. Department of Molecular Biology, Institute for Biological Research, Despot Stephen Boulevard 142, 11060 Belgrade, Serbia and Montenegro
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  • S. Ivanović-Matić,

    1. Department of Molecular Biology, Institute for Biological Research, Despot Stephen Boulevard 142, 11060 Belgrade, Serbia and Montenegro
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  • D. Bogojević

    Corresponding author
    1. Department of Molecular Biology, Institute for Biological Research, Despot Stephen Boulevard 142, 11060 Belgrade, Serbia and Montenegro
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Corresponding author. Tel.: +381 11 2078301; fax: +381 11 2761 433. dekana@ibiss.bg.ac.yu

Abstract

Interaction between transcription factor p53 and the hormone response element (HRE) of the haptoglobin (Hp) gene in adult rat liver was studied. We detected a sequence homologous to the p53 consensus DNA-binding site in the regulatory promoter element of the Hp gene. DNA-affinity chromatography, followed by Western immunoblot analysis with an antibody to p53 indicated that components of the nuclear extract possessed the same antigen determinants as p53. While p53 was identified in both control and acute-phase (AP) samples, DNA-binding affinity for the Hp gene HRE was detected only in the nuclear extract prepared from rats undergoing the AP response. Whether either as an inducible or as a constitutive transcription factor, p53 could be involved in the transcriptional regulation of the Hp gene in adult rat liver.

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