5-Azacytidine decreases fragmentation of nuclear DNA and pigment formation in first leaf cells of barley seedlings
Version of Record online: 2 JAN 2013
© The Author(s) Journal compilation © 2005 International Federation for Cell Biology
Cell Biology International
Volume 29, Issue 12, pages 1025–1031, December 2005
How to Cite
Shkute, N. and Stivrina, N. (2005), 5-Azacytidine decreases fragmentation of nuclear DNA and pigment formation in first leaf cells of barley seedlings. Cell Biology International, 29: 1025–1031. doi: 10.1016/j.cellbi.2005.10.010
- Issue online: 2 JAN 2013
- Version of Record online: 2 JAN 2013
- DNA fragmentation;
- Barley seedlings
Realization of programmed cell death in senescence represents an activation/inactivation of the respective gene. Enzymatic methylation of nuclear DNA with the creation of 5methylcytosine is one of the mechanisms, which can regulate gene activity in animal and plant cells. 5azacytidine (5azaC) acts as an inhibitor of DNA methylation, and induces expression of a range of some genes including genes responsible for senescence. Fragmentation of nuclear DNA is one of the hallmarks of programmed cell death in apoptosis pathway in plant cells. The influence of 5azaC (100 μkg/ml) on nuclear DNS amount and its fragmentation in the first leaf cells of barley was studied. It was shown that in the first leaf cells of barley seedlings there is an apoptosis pathway of programmed cell death. It was also observed that nuclear DNA fragmentation under the 5azaC influence is strongly inhibited, and the DNA amount in the first leaf increases.
Synthesis and destruction of chlorophyll also play a significant role in programmed cell death in plants. It was shown that under the 5azaC influence, the absorption spectrum of a pigment does not change in leaves and coleoptiles in the light, whereas in the dark condition, these pigments are not created under the 5azaC influence.