Both authors contributed equally to this work.
Effect of mouse Sim2 gene on the cell cycle of PC12 cells
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 2006 International Federation for Cell Biology
Cell Biology International
Volume 30, Issue 4, pages 349–353, April 2006
How to Cite
Meng, X., Shi, J., Peng, B., Zou, X. and Zhang, C. (2006), Effect of mouse Sim2 gene on the cell cycle of PC12 cells. Cell Biology International, 30: 349–353. doi: 10.1016/j.cellbi.2005.11.012
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Received 25 July 2005, revised 1 November 2005, accepted 30 November 2005
- Down syndrome;
- Cell cycle;
- Cyclin E;
Sim2 gene plays an important role in the pathogenesis of Down syndrome (DS). To observe the effect of mouse Sim2 (mSim2) on the cell cycle of PC12 cells in vitro and explore the role of Sim2 in the pathogenesis of DS, we cloned the full open reading frame of mSim2 into the pcDNA3 vector and transfected it into PC12 cells, before analysing the effect of mSim2 on the cell cycle. A eukaryotic expression vector of mSim2 (pcDNA3-mSim2) was successfully constructed. There was notable expression of mSim2 mRNA in the cells transfected with pcDNA3-Sim2. Flow cytometry showed that there were more cells in G0/G1 phase in the Sim2-transfected cells than that in the controls (P < 0.01), and significantly fewer in G2/M phase (P < 0.01). The mRNA and protein expressions of cyclin E decreased in the Sim2-transfected cells, while p27 expression increased significantly (P < 0.01). It is concluded that Sim2 may play an important role in the pathogenesis of DS by inhibiting the cell cycle, which is related to the decreased expression of cyclin E and increased expression of p27.