Morphological and growth alterations in Vero cells transformed by cisplatin

Authors

  • Estela Maria Gonçalves,

    Corresponding author
    1. Department of Cell Biology, Institute of Biology, State University of Campinas (UNICAMP), P.O. Box 6109, 13084-971 Campinas, São Paulo, Brazil
      Corresponding author. Tel./fax: +55 19 3788 6111. sgenari@nutricell.com.br
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  • Cláudio Ângelo Ventura,

    1. Department of Biochemistry, Institute of Biology, State University of Campinas (UNICAMP), P.O. Box 6109, 13084-971 Campinas, São Paulo, Brazil
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  • Tomomasa Yano,

    1. Department of Microbiology, Institute of Biology, State University of Campinas (UNICAMP), P.O. Box 6109, 13084-971 Campinas, São Paulo, Brazil
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  • Maria Lígia Rodrigues Macedo,

    1. Department of Biochemistry, Institute of Biology, State University of Campinas (UNICAMP), P.O. Box 6109, 13084-971 Campinas, São Paulo, Brazil
    2. Department of Natural Sciences, State University of Mato Grosso do Sul (UFMS), Três Lagoas, Mato Grosso do Sul, Brazil
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  • Selma Candelária Genari

    1. Department of Cell Biology, Institute of Biology, State University of Campinas (UNICAMP), P.O. Box 6109, 13084-971 Campinas, São Paulo, Brazil
    2. Regional University Center of Espírito Santo do Pinhal (CREUPI), Espírito Santo do Pinhal, São Paulo, Brazil
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Corresponding author. Tel./fax: +55 19 3788 6111. sgenari@nutricell.com.br

Abstract

Cisplatin is an antineoplastic agent used to treat solid tumours, such as ovarian, testicular and bladder tumours. However, studies in vitro and in vivo have shown that cisplatin is mutagenic, genotoxic and tumorigenic in other tissues and organs. In this work, we examined the effect of cisplatin on Vero cells, a fibroblast-like cell line. The morphological characteristics were investigated using phase contrast microscopy, scanning electron microscopy and the actin cytoskeleton was labelled with fluorescein isothiocyanate-phalloidin. Cell proliferation was assessed based on the growth curve. Cultured Vero cells treated with cisplatin showed behavioural and morphological alterations associated with cellular transformation. The transformed cells grew in multilayers and formed cellular aggregates. The proliferation and morphological characteristics of the transformed cells were very different from those of control ones. Since transformed Vero cells showed several characteristics related to neoplastic growth, these cells could be a useful model for studying tumour cells in vitro.

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