These authors contributed equally to this work.
Factors derived from mouse embryonic stem cells promote self-renewal of goat embryonic stem-like cells
Version of Record online: 2 JAN 2013
© The Author(s) Journal compilation © 2006 International Federation for Cell Biology
Cell Biology International
Volume 30, Issue 5, pages 452–458, May 2006
How to Cite
Tian, H.-B., Wang, H., Sha, H.-Y., Xu, X.-J., Zhu, M., Wu, Y.-B., Cheng, S.-H., Chen, J.-Q., Shi, Y.-X., Bai, Z.-L. and Cheng, G.-X. (2006), Factors derived from mouse embryonic stem cells promote self-renewal of goat embryonic stem-like cells. Cell Biology International, 30: 452–458. doi: 10.1016/j.cellbi.2006.02.006
- Issue online: 2 JAN 2013
- Version of Record online: 2 JAN 2013
- Received 29 October 2005, revised 8 January 2006, accepted 14 February 2006
- Goat embryonic stem-like cells;
- Mouse ES cell conditioned medium;
- Inner cell masses;
- Feeder layer;
Goat embryonic stem (ES)-like cells could be isolated from primary materials-inner cell masses (ICMs) and remain undifferentiated for eight passages in a new culture system containing mouse ES cell conditioned medium (ESCCM) and on a feeder layer of mouse embryo fibroblasts (MEFs). However, when cultured in medium without mouse ESCCM, goat ES-like cells could not survive for more than three passages. In addition, no ES-like cells could be obtained when ICMs were cultured on goat embryo fibroblasts or the primary materials-whole goat blastocysts were cultured on MEFs. Goat ES-like cells isolated from ICMs had a normal karyotype and highly expressed alkaline phosphatase. Multiple differentiation potency of the ES-like cells was confirmed by differentiation into neural cells and fibroblast-like cells in vitro. These results suggest that mouse ES cells might secrete factors playing important roles in promoting goat ES-like cells' self-renewal, moreover, the feeder layers and primary materials could also influence the successful isolation of goat ES-like cells.