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Differential expression of connexin 43 in mouse mammary cells
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 2006 International Federation for Cell Biology
Cell Biology International
Volume 30, Issue 5, pages 472–479, May 2006
How to Cite
Lambe, T., Finlay, D., Murphy, M. and Martin, F. (2006), Differential expression of connexin 43 in mouse mammary cells. Cell Biology International, 30: 472–479. doi: 10.1016/j.cellbi.2006.02.008
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Received 18 January 2005, revised 7 April 2005, accepted 11 July 2005
- Gene expression;
- Mouse mammary gland;
- Connexin 43;
- Primary mammary cell culture
In this study we have employed suppressive subtractive hybridization (SSH) analysis to investigate differential gene expression in primary mouse mammary epithelial cells (PMMEC) cultured under mildly apoptotic/quiescent and differentiating conditions. Among a small group of genes whose expression was differentially regulated was connexin 43. In vitro, connexin 43 mRNA and protein were detectable in PMMEC cultured under proliferative or mildly apoptotic conditions. The level of connexin 43 mRNA expression in vivo was also investigated. High levels of expression were found to be associated with the periods of greatest glandular plasticity (pubertal expansion of the mammary tree, early pregnancy and during early involution). Thus, terminally differentiated cells in vivo and in vitro did not express connexin 43 mRNA suggesting that connexin 43 expression, and perhaps facilitated gap junction communication, is associated with undifferentiated progenitor cell populations.