αII-Spectrin interacts with five groups of functionally important proteins in the nucleus

Authors

  • Deepa M. Sridharan,

    1. Department of Pathology and Laboratory Medicine, UMDNJ—New Jersey Medical School and the Graduate School of Biomedical Sciences, Newark, NJ 07103, USA
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  • Laura W. McMahon,

    1. Department of Pathology and Laboratory Medicine, UMDNJ—New Jersey Medical School and the Graduate School of Biomedical Sciences, Newark, NJ 07103, USA
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  • Muriel W. Lambert

    Corresponding author
    1. Department of Pathology and Laboratory Medicine, UMDNJ—New Jersey Medical School and the Graduate School of Biomedical Sciences, Newark, NJ 07103, USA
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Corresponding author. Tel.: +1 973 972 4405; fax: +1 973 972 7293. mlambert@umdnj.edu

Abstract

Nonerythroid α-spectrin (αSpIIΣ*) is a structural protein that has been identified in the nucleus of mammalian cells and shown to be involved in DNA repair. It is also deficient in cells from the clinically diverse genetic disorder Fanconi anemia (FA). In order to get a clearer understanding of the role of αSpIIΣ* in DNA repair, and whether it may have other important functions in the nucleus, studies were undertaken to identify specific αSpIIΣ* protein binding partners in the nucleus. The results demonstrate that multiple proteins co-immunoprecipitate with αSpIIΣ* from nuclear extracts from normal human lymphoblastoid and HeLa cells. These can be grouped into five categories: structural proteins, proteins involved in DNA repair, chromatin remodeling proteins, FA proteins, and transcription and RNA processing factors. These studies indicate that αSpIIΣ* may play a role in a number of diverse and important processes in the nucleus and that a deficiency in this protein, as occurs in FA, could affect a number of critical cellular pathways.

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