Contract grant sponsor: Russian Foundation for Basic Research; Contract grant number: 03-04-48251.
Contribution of α2β1, α3β1, α6β4 integrins and 67 kDa laminin receptor to the interaction of epidermoid carcinoma A-431 cells with laminin-2/4
Version of Record online: 2 JAN 2013
© The Author(s) Journal compilation © 2006 International Federation for Cell Biology
Cell Biology International
Volume 30, Issue 10, pages 784–792, October 2006
How to Cite
Cherepanova, O. A., Kalmykova, N., Petrov, Y. P., Blinova, M. and Pinaev, G. (2006), Contribution of α2β1, α3β1, α6β4 integrins and 67 kDa laminin receptor to the interaction of epidermoid carcinoma A-431 cells with laminin-2/4. Cell Biology International, 30: 784–792. doi: 10.1016/j.cellbi.2006.06.012
- Issue online: 2 JAN 2013
- Version of Record online: 2 JAN 2013
- Received 4 January 2006, revised 4 May 2006, accepted 23 June 2006
- 67 kDa Laminin receptor;
- A-431 cells
Laminin-2/4 is the major laminin isoform of normal muscle and nerve tissues and plays an important role in tumor invasion and metastasis. Despite the fact that laminin-2/4 has been found in the skin basement membrane, insufficient evidence is available on the effect of laminin-2/4 on the behavior of both normal and transformed skin cells. A comparison of the contribution of α2β1, α3β1, α6β4 integrins and 67 kDa laminin receptor on the surface of the human epidermoid carcinoma cell, A-431, to interaction with laminin-2/4 was carried out. The cell interaction with extracellular matrix component is a multistage process. We employed new methods for studying different stages of the interaction of A-431 cells with laminin-2/4. We demonstrated that integrins α2β1, α3β1, α6β4 and 67 kDa laminin receptor are involved in the interaction of A-431 cells with laminin-2/4. We found that contribution of the same receptors to different stages of the interaction with laminin can be different. α2β1 integrins are involved in EGF-induced A-431 cells' migration on laminin-2/4. We demonstrated the cooperation between α2β1 and α3β1 integrins during adhesion and spreading of A-431 cells on laminin-2/4-coated substrate. These results provide information about laminin-2/4 receptors and their contribution to different stages of the interaction with cells.