vasa-related genes and their expression in stem cells of colonial parasitic rhizocephalan barnacle Polyascus polygenea (Arthropoda: Crustacea: Cirripedia: Rhizocephala)

Authors

  • Andrey I. Shukalyuk,

    Corresponding author
    1. Institute of Marine Biology, Far Eastern Branch of Russian Academy of Sciences, Laboratory of Embryology, 17, Pal'chevskogo Street, Vladivostok 690041, Russia
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  • Kseniya A. Golovnina,

    1. Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10, Lavrent'eva Street, Novosibirsk 630090, Russia
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  • Sergei I. Baiborodin,

    1. Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10, Lavrent'eva Street, Novosibirsk 630090, Russia
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  • Konstantin V. Gunbin,

    1. Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10, Lavrent'eva Street, Novosibirsk 630090, Russia
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  • Alexander G. Blinov,

    1. Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10, Lavrent'eva Street, Novosibirsk 630090, Russia
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  • Valeria V. Isaeva

    1. Institute of Marine Biology, Far Eastern Branch of Russian Academy of Sciences, Laboratory of Embryology, 17, Pal'chevskogo Street, Vladivostok 690041, Russia
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Corresponding author. Present address: University of Toronto, Stem Cell Genomics Laboratory, Rosebrugh Building, Room no. 407, 164, College Street, Toronto, ON M5S 3G9, Canada. Tel.: +7 4232 310 924; fax: +7 4232 310 900. work@shukalyuk.net

Abstract

vasa (vas)-related genes are members of the DEAD-box protein family and are expressed in the germ cells of many Metazoa. We cloned vasa-related genes (PpVLG, CpVLG) and other DEAD-box family related genes (PpDRH1, PpDRH2, CpDRH, AtDRHr) from the colonial parasitic rhizocephalan barnacle Polyascus polygenea, the non-colonial Clistosaccus paguri (Crustacea: Cirripedia: Rhizocephala), and the parasitic isopodan Athelgis takanoshimensis (Crustacea: Isopoda). The colonial Polyascus polygenea, a parasite of the coastal crabs Hemigrapsus sanguineus and Hemigrapsus longitarsis was used as a model object for further detailed investigations. Phylogenetic analysis suggested that PpVLG and CpVLG are closely related to vasa-like genes of other Arthropoda. The rest of the studied genes form their own separate branch on the phylogenetic tree and have a common ancestry with the p68 and PL10 subfamilies. We suppose this group may be a new subfamily of the DEAD-box RNA helicases that is specific for parasitic Crustacea. We found PpVLG and PpDRH1 expression products in stem cells from stolons and buds of internae, during asexual reproduction of colonial P. polygenea, and in germ cells from sexually reproducing externae, including male spermatogenic cells and female oogenic cells.

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