Differential expression of phospholipases D1 and D2 in mouse tissues
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 2007 International Federation for Cell Biology
Cell Biology International
Volume 31, Issue 2, pages 148–155, February 2007
How to Cite
Kim, H., Lee, J., Kim, S., Shin, M. K., Min, D. S. and Shin, T. (2007), Differential expression of phospholipases D1 and D2 in mouse tissues. Cell Biology International, 31: 148–155. doi: 10.1016/j.cellbi.2006.09.020
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Received 11 July 2006; revised 16 August 2006; accepted 26 September 2006
- Phospholipase D;
- Tissue distribution;
The differential expression of phospholipase D (PLD) isozymes, which include PLD1 and PLD2, was examined in various murine tissues, including the cerebrum, cerebellum, heart, lung, liver, spleen, stomach, pancreas, ileum, colon, adrenal gland, kidneys, testes, ovaries, and uterus.
In Western blot analysis, only PLD1 was detected in the heart and ovary, while only PLD2 was detected in the pancreas and ileum. Both PLD1 and PLD2 were strongly expressed in the cerebrum, cerebellum, and lung, and both were also expressed in the liver, spleen, stomach, colon, kidney, testes, and uterus.
Immunohistochemistry showed intense PLD immunostaining in the cerebrum, cerebellum, lungs, intestines, and testis, and weak PLD immunostaining in the liver, kidneys, spleen, and heart.
These findings suggest that PLD1 and PLD2 are differentially expressed in the various organs of mice, and that each PLD isozyme plays a distinct role in each organ.