Human umbilical cord blood serum can replace fetal bovine serum in the culture of mesenchymal stem cells

Authors

  • P. Shetty,

    Corresponding author
    1. Haematopoietic Stem Cell Group, Reliance Life Sciences Pvt Ltd, Dhirubhai Ambani Life Sciences Centre, R-282 TTC Industrial Area of MIDC, Thane Belapur Road, Rabale, Navi Mumbai 400 701, India
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    • These authors contributed equally to this paper.

  • K. Bharucha,

    1. Haematopoietic Stem Cell Group, Reliance Life Sciences Pvt Ltd, Dhirubhai Ambani Life Sciences Centre, R-282 TTC Industrial Area of MIDC, Thane Belapur Road, Rabale, Navi Mumbai 400 701, India
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    • These authors contributed equally to this paper.

  • V. Tanavde

    1. Haematopoietic Stem Cell Group, Reliance Life Sciences Pvt Ltd, Dhirubhai Ambani Life Sciences Centre, R-282 TTC Industrial Area of MIDC, Thane Belapur Road, Rabale, Navi Mumbai 400 701, India
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Corresponding author. Tel.: +91 22 6767 8436; fax: +91 22 6767 8099. prathibha_shetty@relbio.com

Abstract

The potential of mesenchymal stem cells (MSC) to differentiate into different cell types has opened up the possibility of using these cells clinically to treat a variety of disorders. In this study we describe the use of human umbilical cord blood serum (CBS) as a replacement for fetal bovine serum (FBS) for culturing MSC from different sources. MSC from human and swine bone marrow and human umbilical cord blood were cultured in the presence of DMEM/F12 containing either FBS or CBS. Human MSC cultured in presence of FBS or CBS showed typical fibroblast-like morphology, which is characteristic of MSC. 99% of the cells cultured in FBS had a CD73+/CD105+/CD45− phenotype compared to 96% of cells cultured in CBS. Cells cultured in CBS had a significantly higher cell count as compared to cells cultured in FBS. Swine Bone Marrow MSC cultured in the presence of FBS and CBS were morphologically and phenotypically similar. Human umbilical cord blood serum supports the growth of MSC. While no significant differences were observed in the MSC numbers in swine cells cultured in the presence of FBS or CBS, human cells showed a greater proliferation potential in the presence of CBS as compared to FBS. Therefore, CBS can be used as an effective substitute to FBS for developing clinically useful protocols for culturing MSC.

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