Apoptosis affects integration frequency: Adult stem cells injected in blastocysts show high caspase-3 activity
Article first published online: 2 JAN 2013
© The Author(s) Journal compilation © 2007 International Federation for Cell Biology
Cell Biology International
Volume 31, Issue 5, pages 489–493, May 2007
How to Cite
Koestenbauer, S., Vanderzwalmen, P., Hammer, A., Schoonjans, L., Danloy, S., Zech, H., Dohr, G. and Zech, N.H. (2007), Apoptosis affects integration frequency: Adult stem cells injected in blastocysts show high caspase-3 activity. Cell Biology International, 31: 489–493. doi: 10.1016/j.cellbi.2006.11.023
- Issue published online: 2 JAN 2013
- Article first published online: 2 JAN 2013
- Received 4 October 2006; revised 30 October 2006; accepted 16 November 2006
- Hematopoietic stem cells;
- Blastocyst injection
Chimeric organisms are commonly generated by injecting stem cells into blastocysts. Embryonic stem cells injected into the blastocoel cavity participate in the further development of the embryo. Adult stem cells have also been used in injection experiments to study their potential plasticity.
In this study we focused on the early fate of injected human adult hematopoietic stem cells (HSCs). HSCs were followed immunohistochemically 1–19 h after injection into murine blastocysts. We found that they only rarely attached and integrated into the blastocysts. The high rate of loss of injected cells after prolonged in vitro culture of the chimeras can be explained by apoptosis. Our findings are consistent with previous studies reporting a low rate of integration of adult cells injected to produce chimeric embryos, but this is the first demonstration that the low efficiency of adult stem cell injections into blastocysts is influenced by apoptosis.