Cytogenetic analysis of human bone marrow-derived mesenchymal stem cells passaged in vitro


Corresponding author. Department of Surgery, University of British Columbia, Rm 400 828 West 10th Ave., Vancouver, BC, Canada V5Z 1L8. Tel.: +1 604 875 4111x62501; fax: +1 604 875 4376.


This study concerns the cytogenetic stability of in vitro human bone marrow-derived mesenchymal stem cells (MSCs) in primary culture and after passaging. Bone marrow samples were collected from seven brain malfunction patients involved in autologous MSC transplantation trials. Chromosome preparations from primary MSC cultures and after 3 passages were analyzed by conventional staining and G-banding techniques. All MSCs showed normal diploid karyotypes, 46 XY or 46 XX, without aneuploidy or polyploidy; chromosome structural abnormalities were not detected. The results indicate that the in vitro cultured MSCs retained normal cytogenetics before being transplanted back into the patients.